Effects of Aphidicolin on Arylsulfatase Gene Expression in Sea Urchin Embryos. (arylsulfatase/aphidicolin/gene expression/sea urchin embryo)
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چکیده
منابع مشابه
The effects of aphidicolin on morphogenesis and differentiation in the sea urchin embryo.
Aphidicolin, an inhibitor of DNA polymerase alpha, blocks DNA synthesis and cell division in sea urchin embryos. The effects of this inhibition appear to be stage dependent. Blastulae treated with aphidicolin before the thickening of the vegetal plate undergo developmental arrest prior to gastrulation. The extent of inhibition of DNA synthesis varies from 60 to 93% in these embryos. However, wh...
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Heterotrimeric kinesin-II is a plus end– directed microtubule (MT) motor protein consisting of distinct heterodimerized motor subunits associated with an accessory subunit. To probe the intracellular transport functions of kinesin-II, we microinjected fertilized sea urchin eggs with an anti–kinesin-II monoclonal antibody, and we observed a dramatic inhibition of ciliogenesis at the blastula sta...
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The developmental course of activity changes of 4 endogenous membrane glycosyltransferases: UDP-glucose : glycosyltrasferase, UDP-galactose : glycosyltransferase, GDP-mannose: glycosyltransferase, and UD-PN-acetylglucosamine : glycosyltransferase was determined in various stages of developing sea-urchin embryos from unfertilized eggs up to the 16-cell stage. The results show that there is a 2-f...
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The presence of fibronectin in developing sea urchin embryos was studied uing immunofluorescence staining. The fluorescence pattern indicates that fibronectin is found on the cell surfaces and between cells in the blastula and gastrula stages, indicating that it plays a role in cell adhesion. Its presence on invaginating cells also suggests its involvement in morphogenesis during early developm...
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ژورنال
عنوان ژورنال: Development, Growth and Differentiation
سال: 1990
ISSN: 0012-1592,1440-169X
DOI: 10.1111/j.1440-169x.1990.00299.x